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REGULATORY MODIFICATION OF THE LIVING ENVIRONMENT FOR RISGENESIS OF PLANTS OF WINTER RYE IN CULTURE IN VITRO

Author(s) Ryabovol Y.S., , postgraduate, Uman National University of Horticulture, Ukraine
Ryabovol L.O., Doctor of Agricultural Sciences, Professor, Professor of Department of Genetics, Plant Breeding and Biotechnology, Uman National University of Horticulture, Ukraine
Category Selection
year 2017 issue №2,2017
pages 64-67 index UDK 631.527.581.143:633.14
DOI
Abstract In the selection process, particular attention is pay to the source material as a source of new economic and valuable features. To find and create new forms it is expedient to use modern biotechnological methods. The article presents the results of studies of stimulation of the conditions of induction of rhizogenesis and plant rooting of valuable samples of winter rye in culture in vitro using microcloning as a method for obtaining a genetically identical material when multiplying self-compatible forms of culture. An environment for inducing the development of the root system of winter rye plants was developed. The influence of exogenous auxins and gibberellins on rooting of biomaterial is determined. The concentration of 1.0 mg / L of indolylacetic acid and 0.5 mg / L of gibberellic acid in the modified nutritional medium Murassigu-Skooga is optimal for the formation and intensive growth of the roots of the biomaterial for microclonal propagation of plants has been shown. The average of 96.3% of samples formed a branched root system, when using the medium. The rhizogenesis of the cloned material is a genetically determined factor has been established.
Key words winter rye, genotype, rhizogenesis, nutrient medium, gibberellic acid.
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