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Sterilization of sugar beet plant material, depending on the type of sterilizer, concentration and exposure of sterilization in vitro

Author(s) Polishchuk V. V., Doctor of Agricultural Sciences, Professor of Department of Landscape Architecture, Uman National University of Horticulture, Ukraine
Karpuk L. M. , PhD of Agricultural Sciences, Associate Professor of Agricultural Chemistry and Soil Science Department, Bila Tserkva National Agrarian University
Pushka I.M., Candidate of Agricultural Sciences, Assistant Professors of Department of Landscape Architecture, Uman National University of Horticulture, Ukraine
Serzhuk O. P. , PhD of Agricultural Sciences, Associate Professor of Genetics, Plant Breeding and Biotechnology Department, Uman National University of Horticulture
Polishchuk T. V. , PhD of Agricultural Sciences, Senior Research Fellow, Institute of Bioenergy Crops and Sugar Beet NAAS of Ukraine
Category Seed production
year 2015 issue №1
pages 71-76 index UDK 633.63:631.52
DOI
Abstract The thesis being presented are results of researches regarding the selection of sterilizer, exposure of sterilization and its concentration in sugar beet hybrids when injected into the culture in vitro. The most effective sterilizing agent to enter micro engraftments in isolated culture is defined 0.1% matched aqueous solution of dichloride mercury exposure for 15 minutes is established. The exit of sterile viable explants from which soon evolved the plants-regenerates is amounted up to 96%. It should be noted that the best sterilizer was the five percent solution of Septodor-forte, for which the increasing of exposure the sterilization from 10 to 20 minutes is obtained the exit of sterile material at 50-90% level.
Key words explants, sugar beet, culture in vitro, sterilization, concentration, exposure of sterilization, plants-regenerates
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