Uman NUH | today: 09/30/2020

PECULIARITIES OF DETECTION OF PATHOGENIC BACTERIA AND CONTROL OF THE DISTRIBUTION IN THE BIOTECHNOLOGICAL PROCESS OF MUSHROOM CULTIVATION

Author(s) Ivanova T. V. , PhD of Agricultural Sciences, , National University of Life and Environmetal Science of Ukraine (Kyiv)
Patyka N. V. , Doctor of Agricultural Sciences, , National University of Life and Environmetal Science of Ukraine (Kyiv)
Tulivetrova K. R. , , , National University of Life and Environmetal Science of Ukraine
Category 202 "Protection and Quaranting of Plants"
year 2020 issue №1, 2020
pages 129-132 index UDK 632.3:606:63:635.8
DOI 10.31395/2310-0478-2020-1-129-132 (Link)
Abstract The article is devoted to a comprehensive study of physiological, biochemical, microbiological, morphological, and cultural features of isolates of pathogenic bacteria during the cultivation of mushrooms. Increasing the production of mushrooms is one of the important issues in the mushroom industry. Industry progress isn`t possible without successfully resolving this issue. At the same time, the practice of the last two years has shown that due to a few economic reasons, the level of industrial mushrooming in our country has decreased. This has led to the accumulation of spent substrates of mushrooming, which causes the infection of pathogens of young mushrooms. The bacterial infections of the mushroom lead to great losses in cultivation. The material of this study was the fruiting body of the mushroom (strain Sylvan 130). Isolated pathogens isolated from mushrooms were the subject of the study. They appeared as primary infections at the stage of active growth of the mushrooms. We used biotechnological research methods in the experiment. Using biochemical methods, we determined the trophic features of pathogenic bacteria. This was done to develop biotechnologies to control their spread. We have studied the physiological and biochemical properties of bacteria, the reaction to the cleavage of carbohydrates on a synthetic medium. The study of the biochemical properties of pathogenic bacteria is necessary to determine their generic and species identity. To detect and study these properties, we cultured the bacteria on nutrient media containing carbon. We also investigated the oxidase activity of bacteria by the Kovacs method and catalase activity. We have scientifically substantiated and proved the ability to recognize the obtained isolates. Based on our research, we have identified the pathogens of this fungus. They were typical only of two-spore mushrooms. We have identified and compared the bacterial properties described above.
Key words pathogenic bacteria, mushrooms, diagnostics, physiological and biochemical properties, identification
Download    (Total: 33)