Uman National University | today: 12/18/2025

ASSESSMENT OF SOIL PHYTOTOXICITY AND MICROBIOLOGICAL POTENTIAL UNDER SYSTEMATIC GREEN MANURING WITH OILSEED RADISH IN CROP ROTATION

Author(s) Ya. H. Tsytsiura, Ph.D. in Agricultural Sciences, Associate Professor, Associate Professor at the Department of Agriculture, Soil Science and Agrochemistry, Vinnytsia National Agrarian University (Vinnytsia, Ukraine)
Category 201 "Agronomy"
year issue
pages 16-29 index UDK 631.5:631.15
DOI 10.32782/2310-0478-2025-2-18-30 (Link)
Abstract The article is devoted to a comprehensive study of the influence of oilseed radish as a green manure crop in crop rotation with stable frequency on the formation of soil phytotoxicity indicators and its microbiological potential. The purpose was to establish an optimal agrotechnological regulation of this green manuring variant and to determine the feasibility and effectiveness of its single-species and uniform application on gray forest soils under conditions of unstable moisture.Over the long-term period of 2014–2025, the dynamics of soil phytotoxicity formation in green manure links of crop cultivation were studied using three criteria: germination of the test crop, plant biomass formation, and the integrated index of conditional coumarin units. This allowed identification of the cumulative nature of soil phytotoxicity increase under systematic use of oilseed radish, with a peak phytotoxic effect (PE) for the 0–30 cm soil layer at the end of the assessment cycle averaging 11.20% PE, while for the criterion of plant biomass formation of the test culture – 12.93% PE. The determined parameters and the identified dynamics of soil phytotoxicity formation allow forecasting of the optimal duration of continuous oilseed radish green manuring in periodic crop rotation links.The study also proved the positive and stable effect of oilseed radish as a green manure on improving both the microbiological potential of gray forest soil, with a 38.52% increase in microbial biomass compared to the initial value, and the structure of ecological-functional groups of soil microorganisms. Over the long-term period of green manuring (2014–2024) with a frequency of once every two years on the same field, increases were recorded at the final date in the shares of the following soil microorganisms: nitrogen-fixing bacteria – by 33.07%, phosphate-mobilizing bacteria – by 29.81%, pedotrophs – by 35.17%, ammonifiers – by 23.49%, amylolytic bacteria – by 29.34%, nitrifiers – by 37.33%, and cellulolytic bacteria – by 43.54%.
Key words oilseed radish, intermediate green manuring, phytotoxic effect, soil bioindication, soil microbiotic complex, ecological-functional groups of soil microorganisms
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